The Queen normally worships at the Chapel of All Saints, in the grounds of Royal Lodge, Windsor Great Park, for reasons of privacy, but she attends services at St George's Chapel on certain special occasions. These include the Easter morning service every year. For much of her reign she has attended mattins on Christmas Day in the chapel.

The Queen also attends services on occasions of particular importance such as the celebration of the Chapel Quincentenary in 1975, concerts to mark special anniversaries, the Opening of St George's House on 23 October 1966, the service in celebration of her 60th Birthday on 21 April 1986, and mattins on the 80th Birthday of the Duke of Edinburgh, which fell on a Sunday, 10 June 2001.

The Duke of Edinburgh presides over the annual St George's House lecture, which other members of the Royal Family also attend.

The Prince of Wales was patron of a concert in the chapel in February 2003, commemorating Queen Elizabeth The Queen Mother, and his great-great aunt, Grand Duchess Elisabeth of Russia.

The Duke of Gloucester presides over the Fabric Advisory Committee.

The late Princess Margaret was a regular worshipper at the chapel, frequently attending the Sung Eucharist, when staying at Royal Lodge.

Many royal baptisms and confirmations have taken place in the private chapel in the State Apartments of the Castle, involving the Dean of Windsor and the St George's Chapel choir. HRH Prince Henry of Wales was baptised in St George's in 1984, and HRH Prince William of Wales was confirmed at a service in the chapel in 1997.